MS3 TMT® Mass Spectrometry Workflow

MS3 TMT® Mass Spectrometry Workflow

GREATLY ENHANCED ACCURACY & PRECISION OF MULTIPLEXED QUANTITATIVE MASS SPECTROMETRY
 
A quantum leap in TMT® isobaric mass spectrometry performance exclusive to Proteome Sciences

Proteome Sciences has developed a proprietary, multiplexed quantitative mass spectrometry based workflow that virtually eliminates the ratio distortion interference seen with current tandem mass spectrometry (MS2) methodologies which use isobaric labeling techniques. This represents a significant breakthrough in the evolution of mass spectrometry for use in multiplexed quantitative proteomics, and opens the door to use of this technology in a far wider range of proteomics applications for basic research, drug discovery and diagnostics.

The new MS3 TMT® (three-stage MS Tandem Mass Tag) mass spectrometry workflow yields significantly enhanced quantitative performance and results, and is available only through PS Biomarker ServicesTM.


Applications Sample Types Species Analysis Method
Drug Discovery

All

 All  TMT®-Orbitrap Velos Pro Mass Spectrometry
Clinical Analysis; early disease detection/prognostic

All

 All TMT®-Orbitrap Velos Pro Mass Spectrometry

 

The MS3 TMT® Workflow Offers

  • Increased accuracy and sensitivity of protein quantitation
  • Removes drawback of isobaric tagging of complex samples – MS3 TMT® gives virtually no ratio distortion interference
  • Utilizes proprietary (Tandem Mass Tags®) TMT® isobaric labels for accurate multiplexed mass spectrometry analysis
  • Applicable for all mass spectrometry methods using TMT
  • Uses industry leading Orbitrap Velos Pro mass spectrometer
  • Allows absolute quantitation of protein analytes
  • Large fold protein concentration changes are now accurately reported
  • Suitable for enhancing drug discovery, biomarker discovery, pre-clinical and clinical diagnostic assay applications

MS3 TMT WorkflowMS2 vs MS3 Quantitation

 

Background and Intended Use

The new and exclusive MS3 TMT® workflow is a powerful augmentation of the widely used Tandem Mass Tag isobaric labeling technology developed by Proteome Sciences. The workflow is offered as an option for all assays and applications available through PS Biomarker ServicesTM, and can be applied to any multiplexed mass spectrometry assay which utilizes Tandem Mass Tag (TMT) isobaric labeling

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Use of triple-stage mass spectrometry (MS3) has been shown to overcome the inherent interference of more commonly used two-stage (MS2) workflows when isobaric labeling strategies are used with complex samples to enable multiplexed analyte analysis. Incorporating a third isolation and fragmentation step of the TMT® labeled protein samples, the new workflow all but eliminates the ratio distortion interference effect and enables far greater accuracy of reporting quantitative results.

The new MS3 TMT® protocols can be used across the board for all multiplexed mass spectrometry assays employing TMT® isobaric labels. The workflow is anticipated to be equally useful as both a discovery tool and as a method used in clinical research and diagnostic applications:

Drug Discovery – evaluate efficiency of new chemical entities & identify off-target effects early

Clinical Research – understand more about disease biology and identify potential drugable targets

Diagnostics – monitor disease stage, progression and response to treatment

 

Contact us to learn more about Proteome Sciences’ proprietary MS3 TMT® workflow or to arrange a consultation with our scientists for an optimized or customized solution to your Biomarker Discovery project.

  

To Download the MS3 TMT® Mass Spectrometry Workflow Product Brochure- Click Here