Validation

Biomarker Validation

All Biomarkers Confirmed & Validated Using Orthogonal Assays

 

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Validation of candidate biomarkers is achieved by confirmation of the original result using a different assay or technology. Generally, this involves using an array of immunoassays but validation can also be complemented with various mass spectrometry methodologies.



Technologies used to confirm & validate initial results:

  • ELISA (Enzyme Linked Immunosorbent Assay)
  • Western Blot
  • Immunohistochemistry
  • TMT®-SRM (Tandem Mass Tag Selective Reaction Monitoring) Mass Spectrometry
  • SRM (Selected Reaction Monitoring) Mass Spectrometry
  • AQUA (Absolute Quantification) Mass Spectrometry

Figure 1. (above) - Typical validation work flow.


Our highly experienced team of scientists are available for consultation to determine the optimal validation work plan and testing protocols to use for your specific project.



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 to learn more about the extensive range of Products and Services available through PS Biomarker ServicesTM or to arrange a consultation with our scientists.