Assays for Measurement of Cell Signaling Pathway Activity in Cancer
Within the body, cells constantly ‘talk’ with each other via a complex and myriad system involving numerous chemical messengers and relay systems that communicate signals between the cells of different tissues and organs. This constant ‘chatter’ is essential to maintaining healthy, functioning tissues that grow and repair themselves in a controlled and organized fashion, and respond to outside stimuli and pathogens appropriately. When these communications systems and cell signaling pathways are disrupted, pathogenic processes such as cancer often arise.
Proteome Sciences are developing numerous multiplexed assays intended for measurement of signaling molecules involved in Cell Signaling Pathways, including post-translational modifications such as phosphorylation, and signaling receptors such as Estrogen Receptor, Progesterone receptor and HER2. Click on the link to learn more about the Breast Cancer Pathway 3-Plex Assay for simultaneous measurement of ER, PR and HER2:
In addition to SysQuantTM Global Phosphorylation Assay described below, we are developing a range of targeted assays for key signaling pathway checkpoint proteins – Checkpoint SRM™ and other key molecules involved in signaling events. For more information contact us .
When a signal molecule (receptor ligand) binds to its receptor on the cell surface, a cascade of transferred signals occurs within the cell. Many of the intermediaries in these signaling cascades are phosphoproteins (proteins that can be altered, activated or deactivated by addition of phosphate groups) that are phosphorylated by enzymes called kinases. Indeed many of the phosphoproteins are kinases themselves and most pathways consist of multiple protein kinase molecules that sequentially phosphorylate each other until a signal is elicited in the nucleus that alters gene activation and protein expression.
To Learn More about our SysQuant Global Phosphorylation Assay, please visit our product page.