Tandem Mass Tag® (TMT®) Protein and Peptide Labeling
Proteome Sciences has a long and successful track record in discovering, validating and implementing biomarkers in many different sample types and across a diverse range of indications. At the heart of our approach is the use of the very latest and most sensitive mass spectrometry instrumentation and techniques, coupled with our own proprietary Tandem Mass Tags® that allow tighter control on analytical variability and rapid development of mass spectrometry assays for biomarker validation. TMT® works like a car tracker system but only to monitor proteins and peptides. TMT® protein labeling reagents and kits are commercially available exclusively from Thermo Scientific, and enable isobaric labeling of proteins extracted from cells, tissues and other biological samples for multiplexed quantitative measurement by mass spectrometry. Currently available in duplex and sixplex isobaric sets and a pair of isotopic reagents that all share the same chemical structure TMT’s are uniquely able to bridge between discovery and candidate verification work flows.
TMT’s are the only isobaric reagents offering a choice of site-specific chemistries:
- NHS - primary amines
- cys - free cysteine residues (reversible)
- iodo - free cysteine residues (irreversible)*
- hydrazine - aldehydes & ketones*
Leading proteomic research groups are adopting TMT reagents and recent technology developments provide improved quanitative precision when analysing complex proteomic samples (Ref 1). We continue to expand the range of TMT reagents and an 18-plex set will be available in 2012 for beta-testers.
*Currently only available to beta-testers through Proteome Sciences plc