SysQuant®
SysQuant®
Global Phosphoproteomic Workflow

Next-Generation Phosphoproteomic Services 

SysQuant® delivers actionable knowledge on key biological processes driving disease and response to treatment

Precision Medicine could transform treatment outcomes but requires deeper understanding of disease biology, drug-target interaction and a system-wide view of mechanisms of action and resistance. Global phosphoproteomics profiling of tissues and cells can identify new targets and candidate biomarkers for early diagnosis, drug selection and monitoring of treatment outcomes. Combining TMT®10plex labeling with the fastest, most accurate mass spectrometers, SysQuant® provides the most comprehensive analysis of protein activity across regulatory and signaling pathways.

Applications

Sample Types

Species

Analysis Method

Pre-Clinical Drug Development

Cultured cells, Tumor Xenografts (fresh/ frozen)

All Mammals, Drosophila & Zebrafish

  • Mechanism of Action
  • Mechanism of Resistance
  • Toxicity Profiling
  • Pharmacodynamics
  • Biomarker Discovery

Clinical Drug Development

Tumor biopsy, Patient-Derived Xenograft, Patient Derived Cell Culture (fresh/frozen)

Human

  • Mechanism of Action
  • Mechanism of Resistance
  • Pharmacodynamics
  • Biomarker Validation

Clinical Medicine

Tumor Biopsy, Patient-Derived Xenograft, Patient Derived Cell Culture (fresh/frozen)

Human

  • Optimized Drug Selection
  • Outcome Monitoring
  • Intelligent Drug Switching

The SysQuant® Global Phosphoproteomic Workflow Offers:

  • Unbiased survey of cell signaling pathways regulated by phosphorylation
  • Combined power of TMT®10plex reagents and Orbitrap Fusion Tribrid™ mass spectrometry
  • ~1,000,000 quantitative data points per TMT®10plex
  • >35,000 proteins and ~40,000 phosphorylation sites*
  • >8,000 unique protein groups and >12,000 unique phosphorylation sites*
  • Standardized data outputs in Excel and CSV files
  • Automated annotation of biological features from GO terms, KEGG, Phosphosite Plus, UniProt & Drugbank
  • Common protocol for all sample types
  • Results in 4 weeks (based on 10 sample study)
  • Optional statistical analysis and biological interpretation packages

*The difference between the number of proteins and unique protein groups reflects redundancy in peptide sequences between protein forms. A peptide that is common to the eight isoforms of tau will be counted 8 times for the number of proteins but only once for the number of unique protein groups. It is now widely considered that typical cells/tissues express between 8 and 10,000 protein groups.

Case Studies

Turning Problems...
Into Solutions

Unravelling the Mechanism of Action of Combined Targets in Melanoma
Unravelling the Mechanism of Action of Combined Targets in Melanoma
Product: SysQuant® Global Phosphoproteomics
READ MORE
Translating Experimental Model Data to Human Disease
Translating Experimental Model Data to Human Disease
Product: SysQuant® Global Phosphoproteomics
READ MORE
Putting Personalized Medicine to Work in Pancreatic Cancer
Putting Personalized Medicine to Work in Pancreatic Cancer
Product: SysQuant® Global Phosphoproteomics
READ MORE
Have questions about SysQuant® or other workflows?
Get more information about SysQuant® or arrange a consultation with our scientists for an optimized or customized solution to your Biomarker Discovery project.