TMT®MS3
TMT®MS3
Highest Accuracy Workflow

Multiplexing for More Complex Samples

TMT®MS3 delivers the most accurate quantitative results whilst retaining good coverage in complex samples


TMT®MS3 is the standard method for biomarker discovery in plasma and other highly complex samples where quantitative accuracy is the most important factor. In such samples, quantitation by MS/MS fragmentation can become compromised and a further round of fragmentation (MS3) is required. Whilst using MS3 is slower and so reduces total proteome coverage, the gain in quantitative accuracy can reveal biomarkers that would be missed by other techniques. Typically, the TMT®10plex reagents are used to allow multiplexing across study cohorts. A common study reference is included in all TMT®10plexes to allow measurement and removal of batch effects and calculation of expression ratios.

ApplicationsSample TypesSpeciesOutcomes
Preclinical Drug Development

Cell culture, Tissue biopsy, Xenograft Tumors

Bacteria, Mammal, Insect

  • Target Discovery / Validation
  • Mechanism of Action / Resistance
Clinical Drug Development

Biopsy Tissue, Peripheral Fluids

Human
  • Mechanism of Action / Resistance
Biomarker Discovery

Biopsy Tissue, Peripheral Fluids

Human, Rodent, Other

  • Candidate Biomarker List

TMT®MS3 data is processed through an integrated bioinformatics pipeline providing data integration and pre-processing (DIANA), statistical analysis (FeaST) and functional analysis (FAT) with outputs suitable for further client-led analysis and presentation to management.

The TMT®MS3 Workflow Offers:

  • Unbiased survey of regulated cell biology in cell lysates and tissue samples with the highest quantitative accuracy
  • Unbiased survey of regulated proteins and peptides in complex peripheral fluids with the highest quantitative accuracy
  • Combined power of TMT®10plex reagents and Orbitrap Fusion™ mass spectrometers
  • Can be combined with a wide range of sample preparation techniques including fractionation and enrichment strategies
  • Standardized data outputs in Excel and ‘TSV’ files
  • Automated annotation of biological features from UniProtKB, Entrez IDs and Reactome
  • Results in 4 weeks (based on 10 sample study)
  • Simple report highlighting key biological events and regulated features enriched in the study
  • Suitable for enhancing drug discovery, biomarker discovery, pre-clinical and clinical diagnostic assay applications
Case Studies

Turning Problems...
Into Solutions

Profiling Cancer Plasma Biomarkers
Profiling Cancer Plasma Biomarkers
Product: TMT®MS3 Quantitative Proteomics
READ MORE
Differentiating ALS Endophenotypes Using PBMC Biomarkers
Differentiating ALS Endophenotypes Using PBMC Biomarkers
Product: TMT®MS3 Quantitative Proteomics
READ MORE
Proteomic Screening for Neurotoxic Effects of Pesticides and Environmental Pollutants
Proteomic Screening for Neurotoxic Effects of Pesticides and Environmental Pollutants
Product: TMTcalibrator™ Tissue-Enhanced Fluid Proteomics
Product: TMT®MS3 Quantitative Proteomics
READ MORE
Have questions about TMT®MS3 or other workflows?
Get more information about Proteome Sciences’ proprietary TMT®MS3 workflow or to arrange a consultation with our scientists for an optimized or customized solution to your Biomarker Discovery project.