TMTcalibrator™
TMTcalibrator™
For Better Biomarkers

Tissue-Enhanced Biomarker Discovery

Simultaneous unbiased quantification of tissue and fluid samples to enhance discovery of mechanistic biomarkers

Finding mechanistic biomarkers related to disease processes and/or drug mechanism of action in peripheral body fluids is extremely challenging due to their low abundance. To overcome this limitation we have developed TMTcalibrator™ where diseased or treated tissue can be analyzed in parallel with peripheral fluids using TMT®10plex reagents. Using a high level of tissue protein relative to the body fluids, we trigger the mass spectrometer on disease-related peptides and can readily determine whether the same peptide is also present in the peripheral fluid. By having peripheral fluids from different clinical groups, regulated, mechanistic biomarker candidates can be readily identified and moved into validation studies.

Applications

Calibrator Sample & Purpose

Fluid Sample

Species

Outcomes

Pre-clinical Biomarker Discovery

Cultured cells, tissue materials,Triggering MS/MS

Any peripheral biofluids (plasma, CSF)

Dog, Monkey, Pig

  • Candidate Pharmacodynamic Biomarkers

Clinical Biomarker Discovery

Cultured cells, tissue materials, Triggering MS/MS

Any peripheral biofluids (plasma, CSF)

Human

  • Candidate Pharmacodynamic Biomarkers

TMTcalibrator™ Workflow Offers:

  • Enhanced detection of tissue-derived  biomarkers in body fluids typically not seen in standard MS workflow
  • Four-point calibration curve for statistical assessment of spectrum quality
  • Allows use of disease-related tissue or cells as surrogate biomarker donor to trigger MS and boost signal of equivalent marker in body fluid
  • Can be combined with a wide range of proteomic workflows e.g. phosphopeptide enrichment to truly maximize signal enhancement
  • Standardized data outputs in Excel and ‘TSV’ files
  • Automated annotation of biological features from UniProt KB, Entrez IDs and Reactome
  • Results in 4 weeks (based on a single TMT®10plex of 6 fluid samples plus 4 point calibrator)
  • Simple report highlighting key biological events enriched in the study
Case Studies

Turning Problems...
Into Solutions

Highlighting Pharmacodynamic Biomarkers of AD
Highlighting Pharmacodynamic Biomarkers of AD
Product: SysQuant® Global Phosphoproteomics
Product: TMTcalibrator™ Tissue-Enhanced Fluid Proteomics
READ MORE
Quantification of Tau and Other Low Abundance Brain Markers in CSF
Quantification of Tau and Other Low Abundance Brain Markers in CSF
Product: TMTcalibrator™ Tissue-Enhanced Fluid Proteomics
READ MORE
Differentiating Fast and Slow Progressing ALS
Differentiating Fast and Slow Progressing ALS
Product: TMTcalibrator™ Tissue-Enhanced Fluid Proteomics
READ MORE
Have questions about TMTcalibrator™ or other workflows?
Get more information about TMTcalibrator™ or arrange a consultation with our scientists for an optimized or customized solution to your Biomarker Discovery project.