PBMCs – An Alternative Source of Biomarkers

TMT®MS2 Broadest Coverage Workflow

Goal – Explore PBMC proteome as a rich source of candidate peripheral biomarkers using TMT® quantification on an Orbitrap Velos Pro.

Outcome – Over 4,000 proteins were quantified including many lower abundance proteins. When compared to the human plasma proteome database around half of plasma proteins were also seen in PBMC’s whereas the PBMC proteome was twice as complex as plasma, confirming their value as a source for peripheral biomarker discovery

Collaborators – Internal project

View all case studies