While all samples types, across all therapeutic areas can be analyzed in a TMT®MS3 study we particularly recommend it for plasma biomarker discovery and other highly complex samples where quantitative accuracy is the most important factor.
Sample multiplexing in TMT®MS3 studies is achieved using TMT®10plex. When sample numbers exceed 10 samples, one of the channel is used as a pooled reference bridging channel, such that each TMT® 10plex set contains 9 study samples plus the reference sample.
The standard TMT® labelling protocol requires a minimum of 100 µg total protein per sample. We also request a minimum of 50µg total protein for sample quality control to assess the suitability of samples for analysis.
Yes, TMT® based workflows are compatible with a wide range of proteomic techniques. Please contact us discuss your specific requirements. If you are specifically interested in phosphopeptide enrichment please see our SysQuant® workflow.
TMT®MS3 studies are run on either a UHPLC-Orbitrap Fusion Tribrid or a UHPLC-Orbitrap Velos Pro, both from ThermoScientific. Please contact us discuss your specific requirements.
Yes, it is possible to add an include list to the mass spectrometry method in TMT®MS3 studies. Please contact us discuss your specific requirements.
The standard data output of TMT®MS3 is QuantSheet™, a Microsoft Excel file. We can also provide other formats such as .CSV or .txt on request. Please discuss specific requirements with our sales team.
TMT®MS3 is our standard workflow for plasma biomarker discovery and other highly complex samples where quantitative accuracy is the most important factor. The workflow combines the multiplexing power of tandem mass tags (TMT®), with state of the art mass spectrometry (MS), MS3 fragmentation & bioinformatics to provide the most accurate quantitative results while retaining good coverage.