Frequently Asked Questions
What is TMT®SRM?
TMT ® SRM is a targeted mass spectrometry assay format that enables the rapid validation of biomarker candidates.
Why should I choose a TMT®SRM assay over a SRM assay for candidate biomarker validation?
TMT®SRM is particularly suited to candidates identified in TMT® discovery workflows (SysQuant® , TMTcalibrator™, TMT®MS2, TMT®MS3) providing a faster and less expensive approach to biomarker validation than classical SRM.
Why should I choose a TMT®SRM assay over an antibody based method for candidate biomarker validation?
Development of a TMT®SRM assay offers a bespoke approach to validate your candidates in one single assay without the need to purchase multiple antibodies for Western Blotting or ELISA kits.TMT®SRM is particularly suited to the validation of proteins and post-translational modifications that do not have commercially available antibodies.
How many proteins can be included in a TMT®SRM Assay?
TMT®SRM assays are typically developed for 5 - 50 biomarker candidates.
What are the stages of TMT ® SRM Assay Development?
There are four stages in the TMT ® SRM Assay Development process:
- Phase 1 - Selection of proteotypic peptides
- Phase 2 - TMT ® SRM assay development and validation
- Phase 3 - Biomarker qualification in small cohort
- Phase 4 - Biomarker validation in larger cohort
What types of sample can be analysed using a TMT ® SRM assay?
All sample types including cell and tissue lysates and body fluids such as CSF and plasma across all therapeutic areas can be analyzed in a TMT®SRM Assay.
What performance characteristics are defined during TMT®SRM assay development?
LOD, LOQ, precision and linear working range.
What type of mass spectrometry is used for a TMT®SRM study?
TMT®SRM Assays are developed and ran on a UHPLC-TSQ Vantage Triple Stage Quadrupole LC/MS Mass Spectrometer from Thermo Scientific.
What does the TMT®SRM data output look like?
Quantitative values for each analyte are provided normally in a simple spreadsheet format.
What additional bioinformatics and data interpretation packages are available for a TMT®SRM study?
- PS offers its TMT ® SRM customers a biostatistics consultancy service.
- As a consultancy service the details and outputs are discussed with our customers on a case by case basis, to meet the needs of the customer and are typically charged at a daily rate.